Knocking-down the expression of nucleostemin significantly decreases rate of proliferation of rat bone marrow stromal stem cells in an apparently p53-independent manner
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چکیده
منابع مشابه
Effects of Lithium Chloride on In Vitro Proliferation Rate of Rat Marrow- Derived Mesenchymal Stem Cells
Purpose: To evaluate the effects of lithium chloride on MSCs in vitro expansion rate. Materials and Methods: In this experimental study, bone marrow from 8 rats was plated at 5×105- cells/cm2 in the presence of 1,2,5,7 and 10 mM Lithium chloride and expanded through 3 passages. Twelve days after initiatial culture, the cells of different groups were stained with crystal violet in order to compa...
متن کاملFunctional Inhibition of Nucleostemin Gene-Acoordinator of Self-Renewal Ability-In Bone Marrow Derived Mesenchymal Stem Cells by Rnai Strategy
Purpose: The aim is to downregulate the expression level of NS as an important factor in sustaining stem cells and certain types of cancer cells self-renewal ability in bone marrow derived mesenchymal stem cells by RNAi strategy and investigate the effects of absence of NS in these cells. Materials and Methods: Double strand NS-specific and control siRNA oligos were designed and transfected in...
متن کاملEffect of Lithium Chloride on Proliferation and Bone Differentiation of Rat Marrow-Derived Mesenchymal Stem Cells in Culture
Objective(s) It is believed that the mesenchymal stem cell (MSC) differentiation and proliferation are the results of activation of wnt signaling pathway. On the other hand, lithium chloride is reported to be able to activate this pathway. The objective of this study was to investigate the effect of lithium on in vitro proliferation and bone differentiation of marrow-derived MSC. Materials and ...
متن کاملRepressing of SOX6 and SOX9 in Situ Chondrogenic Differentiation of Rat Bone Marrow Stromal Cells
Introduction: SOX9 is a transcriptional activator which is necessary for chondrogenesis. SOX6 are closely related to DNA-binding proteins that critically enhance its function. Therefore, to carry out the growth plate chondrocyte differentiation program, SOX9 and SOX6 collaborate genomewide. Chondrocyte differentiation is also known to be promoted by glucocorticoids through unknown molecular mec...
متن کامل: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
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ژورنال
عنوان ژورنال: Cell Proliferation
سال: 2008
ISSN: 0960-7722
DOI: 10.1111/j.1365-2184.2007.00505.x